Principle Of Fluorescence Activated Cell Sorting Technique

Fluorescence activated cell sorting of live cells.

Principle of fluorescence activated cell sorting technique.

Fluorescence activated cell sorting facs is a specialized type of flow cytometry. Fluorescence activated cell sorting facs is a specialized type of flow cytometry. The charge is specific to the wavelength of the fluorescent light which allows for differential sorting by those different charges. It provides a method for sorting a heterogeneous mixture of biological cells into two or more containers one cell at a time based upon the specific light scattering and fluorescent characteristics of each cell.

Facs fluorescence activated cell sorting differs from conventional flow cytometry in that it allows for the physical separation and subsequent collection of single cells or cell populations. Facs is useful for applications such as establishing cell lines carrying a transgene enriching for cells in a specific cell cycle phase or studying the transcriptome or genome or proteome of a whole population on a single cell level. This strategy is used when the target cell expresses a specific cell surface molecule. A description of fluorescence activated cell sorting of live cell populations.

Fluorescence activated cell sorting facs is a powerful method to physically select a subset of target cells from a mixed population. In a facs fluorescence by a cell induces the device to put a charge on a droplet of the transporting fluid containing that cell. It provides a method for sorting a heterogeneous mixture of biological cells into two or more containers one cell at a time based upon the specific light scattering and fluorescent characteristics of each cell. Sorting involves more complex mechanisms in the flow cytometer than a non sorting analysis.

By utilizing highly specific antibodies labeled with fluorescent conjugates facs analysis allows us to simultaneously collect data on and sort a biological sample by a nearly limitless number of different parameters. Fluorescent activated cell sorting of environmental samples containing microalgae. The molecules can be receptors or protein glycoprotein or lipid surface antigens.